14-3-3 theta antibody (pSer58)

Catalog No. ABIN361482

This anti-14-3-3 theta antibody is a Rabbit Polyclonal antibody detecting 14-3-3 theta in WB, IHC and ELISA. Suitable for Rat.

Quick Overview for 14-3-3 theta antibody (pSer58) (ABIN361482)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-14-3-3 theta Antibody

Binding Specificity: pSer58

Specificity: Specific for endogenous levels of the ~29 kDa 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus, zebra fish

Characteristics: Our 14-3-3 (Ser58) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects 14-3-3 (Ser58) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser58 of rat 14-3-3, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 Protein

Background:

Synonyms: 14 3 3 antibody, 14 3 3 protein beta antibody, 14 3 3 protein beta/alpha antibody, 14 3 3 protein zeta antibody ,14 3 3 zeta antibody,14-3-3 protein beta/alpha antibody, 14-3-3 protein/cytosolic phospholipase A2 antibody, 1433B_HUMAN antibody, GW128 antibody, HS1 antibody, KCIP 1 antibody, KCIP-1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein delta polypeptide antibody, Tyrosine 3/tryptophan 5 -monooxygenase activation protein zeta polypeptide antibody, YWHAB antibody, YWHAD antibody, YWHAZ antibody

Description Logic: Our 14-3-3 (Ser58) rabbit polyclonal phosphospecific primary antibody is produced . It detects 14-3-3 (Ser58) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: YWHAB

Molecular Weight: 29 kDa

Gene ID: 56011

UniProt: P35213

Pathways: Apoptosis, Myometrial Relaxation and Contraction